Anti-p53 Antibody

Cat: 90001-MM06
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All p53 Reagents
CRO Services
Product recommended

All p53 Reagents
Anti-p53 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-p53 Antibody
Validated applications
WB,ELISA,ELISA(Det)
Species reactivity
Reacts with: Cynomolgus
Specificity
Cynomolgus p53
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Cynomolgus p53 / TP53 protein (Catalog#90001-CNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus p53 / TP53 (rC p53 / TP53; Catalog#90001-CNAE; E3U906; Met 1-Asp 393). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-p53 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
ELISA(Det) 1:5000-1:50000 This antibody will detect Cynomolgus p53 in ELISA pair set (Catalog: # SEK90001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 90001-MM04).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-p53 Antibody (Mouse Monoclonal antibody) Images
Cynomolgus p53 Western blot (WB) 13754

Anti-P53 mouse monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:53 kDa

Observed band size:53 kDa

Anti-p53 Antibody Alternative Names
Anti-p53 Antibody;Anti-TP53 Antibody
p53 Background Information

p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor protein p53
References
  • Bakhrat A, et al. (2010) Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. Apoptosis. 15(12):1425-34.
  • Kurzhals RL, et al. (2011) Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss. PLoS Genet. 7(6):e1002103.
  • Pardi N, et al. (2011) In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. Acta Biol Hung. 62(4):397-412.
  • Wells BS, et al. (2012) Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev Biol. 361(2):263-76.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-p53 Antibody Rabbit Monoclonal WB,ELISA,IP 90001-R014
    Anti-p53 Antibody Mouse Monoclonal ELISA(Cap) 90001-MM04
    Anti-p53 Antibody Rabbit Monoclonal IHC-P,ICC/IF,IF 90001-R002
    Anti-p53 Antibody Rabbit Polyclonal ELISA 90001-RP01
    Anti-p53 Antibody Rabbit Polyclonal ELISA 90001-RP02
    Anti-p53 Antibody Rabbit Polyclonal WB 100035-T32
    info info
    添加购物车成功! 添加购物车失败!