p53 / TP53 Antibody (Antigen Affinity Purified)

p53 / TP53 Antibody ( Antigen Affinity Purified ) Datasheet

p53 / TP53 Antibody Product Information

• Immunogen: Recombinant Cynomolgus p53 / TP53 protein (Catalog#90001-CNAE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Cynomolgus p53 / TP53 (rC p53 / TP53; Catalog#90001-CNAE; E3U906; Met 1-Asp 393). p53 / TP53 specific IgG was purified by Cynomolgus p53 / TP53 affinity chromatography.

p53 / TP53 Antibody Usage Guide

• Specificity: Cynomolgus p53 / TP53
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus P53 in WB. Using a DAB detection system, the detection limit for Cynomolgus P53 is approximately 2 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus P53. The detection limit for Cynomolgus P53 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

p53 / TP53 Antibody Background

Cynomolgus Cellular tumor antigen p53, also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53 and p53, is an important inmulticellular organisms, where it regulates thecell cycleand functions as atumor suppressorthat is involved in preventingcancer. Tumor suppressor p53 (TP53 and p53) has many mechanisms of anticancer function, and plays a role in apoptosis, genomic stability, and inhibition ofangiogenesis. In its anti-cancer role, it works through several mechanisms. Tumor suppressor p53 (TP53 and p53) becomes activated in response to a myriad of stress types, which include but are not limited toDNA damage,oxidative stress, osmotic shock, ribonucleotide depletion, and deregulated oncogene expression. Tumor suppressor p53 (TP53 and p53) regulates the cell cycle. It functions as a tumor suppressor that is involved in preventing cancer. Tumor suppressor p53 (TP53 and p53) acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Tumor suppressor p53 (TP53 and p53) isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

References

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