Mouse MAPK13 Gene cDNA Clone / ORF Clone

MAPK13 cDNA Clone Datasheet

MAPK13 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011950.2
• cDNA Size: 1101
• Gene Synonym: SAPK4; Serk4; Mapk13
• Source: Mouse

MAPK13 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mitogen-activated protein kinase 13 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 660 G/A not causing the amino acid variation.

MAPK13 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MAPK13 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MAPK13 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCCTCA CTCGGAAAAG GGGCTTCTAC AAGCAGGACA TCAACAAGAC TGCCTGGGAG
61       CTACCCAAGA CCTACCTGGC GCCCGCGCAC GTCGGCAGCG GGGCCTATGG AGCGGTGTGC
121      TCGGCCATCG ACAAGCGGAC AGGGGAGAAG GTGGCCATCA AGAAGCTGAG CCGGCCCTTC
181      CAGTCGGAGA TCTTTGCCAA GCGCGCTTAC CGCGAGCTTC TGCTCTTGAA GCACATGCAC
241      CATGAGAACG TCATTGGGCT TCTGGATGTC TTCACCCCTG CGTCTTCCCT TCGGAGCTTC
301      CATGATTTCT ACCTGGTGAT GCCCTTCATG CAGACAGACC TACAGAAGAT CATGGGGATG
361      GAATTCAGCG AGGATAAGGT CCAGTACTTG GTGTACCAGA TGCTCAAAGG TCTAAAGTAC
421      ATCCACTCCG CTGGCATCGT CCACAGGGAC CTGAAACCGG GCAACCTGGC TGTGAATGAA
481      GACTGTGAGC TGAAGATCCT GGACTTTGGG CTGGCCCGCC ACACAGACAC TGAGATGACG
541      GGCTATGTGG TGACCCGCTG GTACCGGGCC CCCGAGGTGA TCCTCAGCTG GATGCATTAC
601      AACCAGACAG TCGACATCTG GTCTGTTGGT TGCATCATGG CAGAAATGCT GACTGGAAAA
661      ACACTCTTCA AGGGCAAGGA CTACCTGGAC CAGCTGACCC AGATCCTGAA AGTGACTGGG
721      GTGCCAGGTG CCGAGTTCGT GCAGAAGCTG AAAGACAAGG CGGCCAAATC CTATATTCAG
781      TCCCTGCCCC AGAGCCCCAA GAAGGATTTC ACACAGCTCT TCCCACGCGC CAGTCCGCAA
841      GCTGCAGACC TGCTCGACAA GATGCTGGAG CTGGATGTGG ACAAGCGTCT GACCGCTGCT
901      CAGGCACTGG CTCACCCCTT CTTTGAACCC TTCCGGGACC CTGAGGAGGA GACAGAGGCC
961      CAGCAGCCTT TTGATGATGC CTTAGAACAT GAGAAACTCA GCGTGGACGA ATGGAAACAA
1021     CACATCTACA AAGAGATCTC AAACTTCAGT CCCATAGCCC GGAAGGACTC ACGGCGACGA
1081     AGTGGCATGA AGCTGCAGTG A

 

Quality Control:cDNA sequencing results is available upon request.