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Myc-tag Protein Production & Purification Service

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Myc-tag (EQKLISEEDL-tag) Protein Production & Purification Service

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
Myc Tag Design: Myc tag (EQKLISEEDL-tag) at N- or C-terminus
Cleavage Site: Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
Purification: Myc-tag (EQKLISEEDL-tag) specific antibody conjugated affinity clumn
Myc-tag Affinity Purification Resin
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 8-12 weeks

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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Myc-tag Protein Expression, Production, and Purification Basics

What is a Myc-tag?

A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.

The peptide sequence of the myc-tag is: EQKLISEEDL (or -Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-).

What is the Molecular Wieght of the Myc-tag?

The Myc-tag contains 10 amino acids, and the molecular weight of the Myc-tag (EQKLISEEDL-tag) peptide is 1202 Da.

Why use a Myc-tag for protein expression and production?

Adding a Myc-tag to a protein's C- or N-terminus can facilitate its detection, isolation, and purification by Myc-tag specific antibodies. This is particularly helpful for proteins without a detection antibody or with unknown cellular location. Sino Biological has developed Myc-tag specific antibodies for ELISA, Western Blotting, Immonofluorecence, and Immunoprecipitation detection of the Myc-tagged protein. It can also be conjugated to agarose beads for Myc-tagged protein affinity purification.

How to Purify Myc-tagged Proteins?

Myc-tagged recombinant protein can be affinity

Myc-tag Protein Production & Purification Service Description

Myc-tag (EQKLISEEDL-tag) has been widely used in recombinant protein expression and production. The Myc-tag allows simple and efficient affinity purification of the tagged protein using Myc-tag specific antibody conjugated to agarose-beads. The Myc-tag (EQKLISEEDL-tag) also can be used for detection in western blot by using a Myc-tag-specific antibody. Myc-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.

Sino Biological has successfully produced many proteins with the Myc-tag and purified with Myc-tag affinity chromatography. We are highly experienced in designing the Myc-tag according to the protein structure and handle any problems arising from protein purification process.

Sino Biological has also developed highly sensitive Myc-tag (EQKLISEEDL-tag) specific antibodies for ...... < Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
-- Custom Protein Production Project Description
-- Description of Risk-sharing Protein Production
-- Description of Risk-free Protein Production Project
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
-- Description of Protein Production Service in Insect Cells
- Protein Production Service in Yeast Expression System
-- Description of Protein Production Service in Yeast
- Protein Production Service in E. coli. Cells
-- Description of Protein Production Service in E. coli.
- Protein Refolding Service / Protein Production Service in E. coli.
-- Description of Protein Refolding & Production Project
- Poly His-tag Protein Production & Purification Service
-- Description of Risk-free Protein Production Project
-- Poly-his-tag Protein Expression, Production, and Purification Basics
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
-- DYKDDDK-tag (FLAG-tag) Protein Production Service Description
-- FLAG-tag Protein Expression, Production, and Purification Basics
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
-- Description of Fc-tag Protein Production & Purification Service
-- IgG-Fc-tag Protein Expression, Production, and Purification Basics
- HA-tag Protein Production & Purification Service
-- HA-tag Protein Production & Purification Service Description
-- HA-tag Protein Expression, Production, and Purification Basics
- Myc-tag Protein Production & Purification Service
-- Myc-tag Protein Production & Purification Service Description
-- Myc-tag Protein Expression, Production, and Purification Basics
- GST-fusion-tag Protein Production & Purification Service
-- GST-tag Protein Production & Purification Service Description
-- GST-tag Protein Expression, Production, and Purification Basics
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"