Human interleukin 8 ORF Expression Clone, untagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10098-M-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

interleukin 8 cDNA Clone Datasheet

interleukin 8 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000584.2
cDNA Size:	300
Gene Synonym:	CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Source:	Human

interleukin 8 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens interleukin 8 DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

interleukin 8 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

interleukin 8 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

interleukin 8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACTTCCA AGCTGGCCGT GGCTCTCTTG GCAGCCTTCC TGATTTCTGC AGCTCTGTGT
61       GAAGGTGCAG TTTTGCCAAG GAGTGCTAAA GAACTTAGAT GTCAGTGCAT AAAGACATAC
121      TCCAAACCTT TCCACCCCAA ATTTATCAAA GAACTGAGAG TGATTGAGAG TGGACCACAC
181      TGCGCCAACA CAGAAATTAT TGTAAAGCTT TCTGATGGAA GAGAGCTCTG TCTGGACCCC
241      AAGGAAAACT GGGTGCAGAG GGTTGTGGAG AAGTTTTTGA AGAGGGCTGA GAATTCATAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine