Anti-CDK4 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CDK4 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CDK4 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CDK4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CDK4 Magnetic Beads-IP Kit Product Description

The Anti-CDK4 magnetic Beads, conjugated with Anti-CDK4 antibody, are used for immuneprecipitation (IP) of CDK4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CDK4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CDK4 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CDK4 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CDK4 Antibody(100873-T46)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human CDK4
Species Reactivity
Human CDK4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CDK4, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CDK4 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CMM3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PSK-J3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CDK4 Background Information

CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product. CDK4 is the ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. CDK4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma.
Full Name
cyclin-dependent kinase 4
References
  • Stepanova L, et al. (1996) Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4. Genes Dev. 10(12):1491-502.
  • Lamphere L, et al. (1997) Interaction between Cdc37 and Cdk4 in human cells. Oncogene. 14(16): 1999-2004.
  • Dai K, et al. (1996) Physical interaction of mammalian CDC37 with CDK4. J Biol Chem. 271(36): 22030-4.
  • Sugimoto M, et al. (1999) Regulation of CDK4 activity by a novel CDK4-binding protein, p34SEI-1. Genes Dev. 13(22):3027-33.
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