Anti-CD74 Magnetic Beads Immunoprecipitation (IP) Kit

Price:
Size:
Number:

Anti-CD74 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD74 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD74 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD74 Magnetic Beads-IP Kit Product Description

The Anti-CD74 magnetic Beads, conjugated with Anti-CD74 antibody, are used for immuneprecipitation (IP) of CD74 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD74 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD74 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD74 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD74 Antibody(11091-RP02)
Immunogen
Recombinant Human CD74 protein (Catalog#11091-H07H)
Species Reactivity
Human CD74 / DHLAG / p33
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD74 extracellular domain (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln 73-Met 232). CD74 specific IgG was purified by human CD74 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD74 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-DHLAGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HLADGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Ia-GAMMAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD74 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.
Full Name
CD74 molecule, major histocompatibility complex, class II invariant chain
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Moynes R, et al. (1997) A marker to distinguish atypical fibroxanthoma from malignant fibrous histiocytoma. Cancer. 79 (11): 2115-24.
  • Gabrielle FA, et al. (2008) Regulation of dendritic cell migration by CD74, the MHC class 2-associated invariant chain. Science. 322 (5908): 1705-10.
  • Leng L, et al. (2003) MIF signal transduction initiated by binding to CD74. JEM.197 (11): 1467-76.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait