Immunohistochemistry / IHC Technology Center

Immunohistochemistry (IHC) is the process whereby antibodies are used to detect proteins (antigens) in cells within a tissue section (for instance liver, pancreas or the heart).
When a tissue sample is passed to a lab to be examined for disease, there are several details that cannot be determined easily. Several diseases or disease sub-types may look alike or appear to have similar size cells under a microscope but have different behaviors and necessary treatments. The best way to differentiate them is to detect specific molecules on these cells that act as markers.
Immunohistochemistry (IHC) is a technique that uses antibodies (matching molecules) that can seek out, identify and attach themselves to these markers on cells. The antibodies themselves can be seen under the microscope, which helps the technician make a precise identification. Immunohistochemistry (IHC) has found numerous applications in medicine, especially in cancer diagnosis. Lymphomas are among the cancers most dependent on IHC for correct diagnosis and treatment decisions.

IHC Introduction

IHC Protocol

IHC Tips

IHC Application

IHC Troubleshooting


Immunohistochemistry / IHC Antibody
Immunohistochemistry / IHC Antibody Features
Immunohistochemistry / IHC Product Center
Immunohistochemistry / IHC Technology Center+
- Immunohistochemical / IHC Introduction
What is immunohistochemistry / IHC?
Immunohistochemistry/IHC Definition
Immunohistochemistry/IHC history
DAB Immunohistochemistry/IHC
Immunohistochemical analysis
Immunohistochemistry/IHC techniques
Immunohistochemistry/IHC principle
Immunohistochemical Methods
Immunohistochemistry/IHC staining
Antigen Retrieval
- Immunohistochemical / IHC Protocol
Immunocytochemistry protocol
Paraffin Section Protocol
Frozen Section Protocol
Antigen Retrieval Protocol
Immuno-staining Protocol
Counterstaining Protocol
Apoptosis TUNEL staining protocol
- Immunohistochemical / IHC Tips
Aims of tissue fixation
As for Dehydration, Paraffin Embedding and Sectioning
Deparaffinization and hydration
Antigen retrieval
Cell permeabilization
Inactivation of endogenous peroxidase and biotins
Antibody diluents
Section washing
DAB developing
- Immunohistochemistry / IHC Application
Immunohistochemistry / IHC in Cancer
Immunohistochemistry / IHC in Neuroscience
Immunohistochemistry /IHC in Drug-discovery
- Immunohistochemical / IHC Troubleshooting
No staining
High background
Non-specific staining
- Immunohistochemical / IHC FAQ
Antigen retrieval should I do PIER or HIER?
How to choose antibody for IHC experiments,the monoclonal or polyclonal antibodies ?
Incubation time of primary antibody
How to avoid non-specific staining?
What is imunohistochemistry?
What is the reason for no staining?
How to solve the high background staining?