Immunohistochemical / IHC Protocol

Do you want immunohistochemistry did not know where to start? We provide you a detailed IHC steps. Many experimental methods were prepared include paraffin sections , frozen sections, and antigen retrieval processes, etc., each step of the experimental process is crucial, direct impact on the success of the experimental results. These experiments protocol may help your experiment go on successfully.

Immunohistochemistry / IHC Antibody
Immunohistochemistry / IHC Antibody Features
Immunohistochemistry / IHC Product Center
Immunohistochemistry / IHC Technology Center+
- Immunohistochemical / IHC Introduction
What is immunohistochemistry / IHC?
Immunohistochemistry/IHC Definition
Immunohistochemistry/IHC history
DAB Immunohistochemistry/IHC
Immunohistochemical analysis
Immunohistochemistry/IHC techniques
Immunohistochemistry/IHC principle
Immunohistochemical Methods
Immunohistochemistry/IHC staining
Antigen Retrieval
- Immunohistochemical / IHC Protocol
Immunocytochemistry protocol
Paraffin Section Protocol
Frozen Section Protocol
Antigen Retrieval Protocol
Immuno-staining Protocol
Counterstaining Protocol
Apoptosis TUNEL staining protocol
- Immunohistochemical / IHC Tips
Aims of tissue fixation
As for Dehydration, Paraffin Embedding and Sectioning
Deparaffinization and hydration
Antigen retrieval
Cell permeabilization
Inactivation of endogenous peroxidase and biotins
Antibody diluents
Section washing
DAB developing
- Immunohistochemistry / IHC Application
Immunohistochemistry / IHC in Cancer
Immunohistochemistry / IHC in Neuroscience
Immunohistochemistry /IHC in Drug-discovery
- Immunohistochemical / IHC Troubleshooting
No staining
High background
Non-specific staining
- Immunohistochemical / IHC FAQ
Antigen retrieval should I do PIER or HIER?
How to choose antibody for IHC experiments,the monoclonal or polyclonal antibodies ?
Incubation time of primary antibody
How to avoid non-specific staining?
What is imunohistochemistry?
What is the reason for no staining?
How to solve the high background staining?