Immunofluorescence / IF / ICC FAQ

Immunofluorescence / IF / ICC FAQ

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- Immunofluorescence / IF / ICC Introduction
What is Immunofluorescence (IF)?
What is Immunocytochemistry (ICC)?
Difference between Immunocytochemistry / ICC and Immunofluorescence / IF
Principle of Immunofluorescence
Advantages of Immunofluorescence / IF/ICC
- Immunofluorescence / IF / ICC Protocol
Immunofluorescence protocol (IF protocol)
Immunocytochemistry protocol (ICC protocol)
Immunofluorescence / IF/ICC-Immunostain protocol
- Immunofluorescence / IF / ICC FAQ
Does the immunefluorescence antibodies be used to other application,WB,IP,IHC,FC or CHIP?
What is the specimen commonly used in immunofluorescence l staining experiments?
What is the difference Immunocytochemistry / ICC and Immunofluorescence / IF?
Why the immune fluorescence result has non-specific staining?
How to select the immune fluorescent antibody?
indirect IF or dierect IF?
What is a good result for membrane protein?
What is a good result for cytoplasm protein?
What is a good result for nuclear protein?
How to choose second antibody for IF?
Which is the best method, IF or IHC ?
- Immunofluorescence / IF / ICC Troubleshooting
Positive Control is negative or very weak
Negative Control is positive
Why the immune fluorescence result is no staining?
Why the immune fluorescence result has high background?
Why the immune fluorescence result has non-specific staining?
High background staining showed false positive or negative results
How to choose second antibody for IF?
How to preserve the immuno fluorescence antibody?
Why the antibody without IF application can't used for IF test?
Reactions are stronger on the edges of the well in comparison to the center
- Immunofluorescence / IF / ICC Tips
Antibodies determine success or failure of IFF/ICC
Cell fixation and permeabilization
Cell Use an appropriate cell density
Cell Multiple staining
Cell Reducing background
-Examples of good or bad IF/ICC Results