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Immunofluorescence / IF/ICC Antibody Features

Sino Biological Inc. is the largest centralized protein manufacturer worldwide. Over 60 bioreactors (>1000L cell culture capacity)support 40 projects in parallel. Less than three years, Sino Biological built up one of the largest protein banks with over 7000 proteins and mAbs in stock and serve clients including the top 10 global pharmaceutical / biotech companies. Prducts like cDNA, Cell Factors and Transfection Reagent also got good word of mouth.Our core supporting team were trained in top Chinese, American, and European universities or multinational harmaceutical / biotech companies thus greatly experienced in protein / antibody research, production, and analysis.

Compared to other leading brands. Sino Bological provides antibodies with higher antigen affinity as low as 5 pg (10-200 fold higher than those from other providers in average). That remarkable feature based on our highly strict QC process and various analytical methods.Sino Biological Inc. provides antibodies that can meet different applications, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA.

We are committed to developing high quality Immunofluorescence / IF / ICC Antibody. Sino Biological’s Immunofluorescence / IF / ICC Antibodies were developed with a rigorous screening and validation process.ALL Immunofluorescence / IF / ICC Antibodies have test image for the user's reference.Our antibodies have been validated by multiple cell types with strict negative controls or FACS, IHC or WB confirmations. We have compared our Immunofluorescence / IF / ICC antibodies and other brand’s Immunofluorescence / IF / ICC antibodies, the results show that our Immunofluorescence / IF / ICC Antibodies have better affinity and sensitivity.

Feature 1Better affinity and sensitivity

Sino Biological's Immunofluorescence / IF / ICC antibodies were compared with other international brand’s antibodies which have the same target proteins. All experiments are conducted under the same conditions. The results show that our Immunofluorescence / IF / ICC Antibodies have Better affinity and sensitivity.

EGFR JAM-A GP73 CD155  
Sino Biological
Inc.
Other brands

Feature 2Extensive validation by multiple cell types

Sino Biological's Immunofluorescence / IF / ICC antibodies were validated with nature cells. The cells used in Immunofluorescence / IF / ICC include multiple tumor cell lines, such as Hela,MCF7, HepG2 and A431,and so on, and include multiple stem cells, such as human embryonic stem cell (HESC) and human mesenchymal stem cell (MSC).

A549 HepG2 Hela MCF-7

Feature 3Strict negative controls

The expression of some target proteins is specific, that is only in some cells, but not in other cells. All the positive cells and negative cells can be used for the detection of the Immunofluorescence / IF / ICC antibody, which can be used to detect and verify the specificity of the Immunofluorescence / IF / ICC antibody.

Negative control Positive

Feature 4In-house confocal technology support

There are two main types of immunofluorescence imaging: fluorescence microscopy and confocal microscopy. The focus of the most core technology is to remove the non focal plane information of a certain thickness specimen by using the spatial filtering technology, the optical microscope (CT) can reduce the interference and improve the imaging quality.

fluorescence
microscopy
Positive

Feature 5Other methods (FACS, IHC or WB)confirmations

There are a variety of detection methods for antibody, and the method can verify each other. The Immunofluorescence / IF / ICC antibodies can be verified by the method of IHC, FAC(FC) and WB. If one result of IHC,FAC(FC) and WB was correct, Immunofluorescence / IF / ICC antibodies were validated that the the Immunofluorescence / IF / ICC antibody is specific to target.

LAMP1 / CD107a Antibody, Mouse MAb  
IF/ICC FC IHC WB

Feature 6Antigen blocking assay

Antigen blocking test is a very effective method to prove the specificity of the antibodies. If the binding of antibodies to the natural antigens can be blocked by the addition of free antigens, it can be shown that the antibody is specific for the specific recognition of the antigen, not nonspecific binding or binding to other antigens. Immunofluorescence / IF / ICC antibody can been confirmed again with antigen blocking. The correct of immunofluorescence staining disappeared after antigen blocking, that mean the Immunofluorescence / IF / ICC antibody has better specificity.

Postivie result Antigen blocking
Antibody
Immunofluorescence / IF / ICC Antibody
- Immunofluorescence / IF/ICC Antibody Features
-- Better affinity and sensitivity
-- Extensive validation by multiple cell types
-- Strict negative controls
-- In-house confocal technology support
-- Other methods (FACS, IHC or WB)confirmations
-- Antigen blocking assay
- Immunofluorescence / IF/ICC Products Center
-- Human Target
-- Mouse Target
-- Other Species
-- CD Molecular
-- Biomarker
-- Biological target
-- Diagnostic
-- Cancer
-- Cytokine
-- Receptor
-- Interleukin
-- Stem cell
-- A431
-- 293
-- MCF7
-- ISKBR3
-- Hela
-- MSC
-- SHSY5Y
-- A549
-- Jurkat
-- HESC
-- HUVEC
-- HepG2
-- HT29
-- PC3
-- CHO
-- RAW264.7
-- mouse splenocytes
-- rat splenocytes
-- Mouse monoclonal antibody
-- Rabbit monoclonal antibody
-- Cell membrane
-- Cytoplasm
-- Nucleus
-- Secreted
-- Mitochondrion
-- Lysosome
-- Golgi
-- HA tag
-- DYKDDDDK tag
-- E-tag
-- GFP tag
-- S-tag
- Immunofluorescence / IF / ICC Technology Center
-- Immunofluorescence / IF / ICC Introduction
-- Immunofluorescence / IF / ICC Protocol
-- Immunofluorescence / IF / ICC FAQ
-- Immunofluorescence / IF / ICC Troubleshooting
-- Immunofluorescence / IF / ICC Tips
- Examples of good or bad IF/ICC Results
-- A431-Cell membrane
-- A431-Cytoplasm
-- A431-Nucleus
-- Hela-Cell membrane
-- Hela-Cytoplasm
-- Hela-Nucleus
-- Hela-Lysosome
-- Hela-Golgi
-- Hela-Mitochondrion
-- Hela-Microtubule
-- A549-Cytoplasm
-- A549-Nucleus
-- A549-Lysosome
-- HepG2-Cytoplasm
-- HepG2-Lysosome
-- HESc-Cytoplasm
-- HESc-Nucleus
-- MCF7-Cell membrane
-- MCF7-Cytoplasm
-- MCF7-Nucleus
-- PC3-Cell membrane
-- SHSY5Y-Cytoplasm
-- SHSY5Y-Nucleus
-- SKBR3-Cell membrane
-- SKBR3-Cytoplasm
-- Raw264.7-Cell membrane
-- Mouse Splenocytes-Cell membrane
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"