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HEK293 Cell Transient Transfection Service

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Transient Transfection Service for Antibody Production

Host Cells: HEK293 cells, 293 Cell, 293F Cells, 293S Cells, CHO cells
Engineered 293 Cells optimized for transient transfection expression up to 12 days
Engineered Chinese Hamster Ovary Cells for optimal antibody production
Technology: Recombinant technology, transient transfection
Stirred-tank cell culture bioreactors
Cell Culture: Serum-free 293 cell or CHO cell culture, fed-batch cell culture
Transfection: Highly efficient transient transfection of suspension HEk293 cells or CHO cells
Transfection reagent: Sinofection Transfection Reagent
Cell Culture Scale: High throughput small scale cell culture at 200 mL to 1 L scale
2L stirred tank cell culture
5L stirred tank cell culture
10L stirred tank cell culture
20L stirred tank cell culture
50L stirred tank cell culture
100L Stirred tank cell culture
300L Stirred tank cell culture
Antibody Purification: Affinity protein A/G/L chromatography column purification
plus ion exchange , SEC chromatography polishing steps as needed
Deliverable: Purified antibody meeting quality specificiations
Service Mode: Cell culture volume based charge or Charge based on deliverable
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas

HEK293 Cell Transient Transfection Service Description

In HEK293 cell transient transfection, large quantities of DNA plasmids containing the target gene of interest are transfected into HEK293 cells, with an efficient transfection reagent that can transport a large number of DNA plasmids into the host HEK293 cells for gene expression.

Sino Biological has developed an engineered HEK293 cell line. The optimized 293 cells can maintain a certain number of DNA copies within the cells for a short period of time, up to 6-12 days. The advantage of transient transfection is that it totally skipped the need for lengthy and costly stable cell line development, and hence it is highly preferable for quick and high-throughput small scale protein and antibody production.

Sino Biological has also optimized the serum-free culture medium for optimal transfection efficiency and hence improved protein expression yield. Furthermore, Sino Biological has developed a highly efficient,........ <Learn More>