|All Antibody Services||All Protein Services||All Gene Services||All Biological Services|
|Full services:||From gene cloning/synthesis to purified proteins
|Host Cells:||Choices of Expression Hosts:
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
|GST Tag Design:||GST tag (Glutathione S-transferases) fused at N- or C-terminus
|Cleavage Site:||Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
|Purification:||Affinity purification by Glutathione Agarose Resin|
|Deliverable:||Purified recombinant proteins (quantities to be specified by customer)|
|Quality Specification:||typically >90% purity by SDS page
|Typical Timeline:||8-12 weeks|
What is a GST-tag?
GST stands for Glutathione S-transferase. It is a family of eukaryotic and prokaryotic metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. It is typically fused to the N-terminus of a recombinant protein through recombinant DNA technology.
The peptide sequence of the GST-tag is 220 amino acids (roughly 26 KDa) in size.
Why use a GST-tag for protein expression and production?
Adding a GST-tag to a protein's N-terminus can ...... < Learn More>
GST-fusion-tag (GST-fusion protein) has been widely used in recombinant protein expression and production. The fusion of the GST protein allows simple and efficient affinity purification of the GST-tagged protein using Glutathione affinity agarose resin. The GST-tag also can be used for detection in western blot by using a GST-tag-specific antibody. GST-tag is typically fused to the N-terminus of a protein for expression in virtually all expression systems, but for purification purposes, it is often used only in E. coli. expression system and baculovirus / insect cell expression system.
Sino Biological has successfully produced many proteins with the GST-tag and ...... < Learn More>