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GST-fusion-tag Protein Production & Purification Service

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GST-fusion-tag Protein Production & Purification Service

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Choices of Expression Hosts:
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
GST Tag Design: GST tag (Glutathione S-transferases) fused at N- or C-terminus
Cleavage Site: Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
Purification: Affinity purification by Glutathione Agarose Resin
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 8-12 weeks

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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GST-tag Protein Expression, Production, and Purification Basics

What is a GST-tag?

GST stands for Glutathione S-transferase. It is a family of eukaryotic and prokaryotic metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. It is typically fused to the N-terminus of a recombinant protein through recombinant DNA technology.

The peptide sequence of the GST-tag is 220 amino acids (roughly 26 KDa) in size.

Why use a GST-tag for protein expression and production?

Adding a GST-tag to a protein's N-terminus can ...... < Learn More>

GST-tag Protein Production & Purification Service Description

GST-fusion-tag (GST-fusion protein) has been widely used in recombinant protein expression and production. The fusion of the GST protein allows simple and efficient affinity purification of the GST-tagged protein using Glutathione affinity agarose resin. The GST-tag also can be used for detection in western blot by using a GST-tag-specific antibody. GST-tag is typically fused to the N-terminus of a protein for expression in virtually all expression systems, but for purification purposes, it is often used only in E. coli. expression system and baculovirus / insect cell expression system.

Sino Biological has successfully produced many proteins with the GST-tag and ...... < Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
-- Custom Protein Production Project Description
-- Description of Risk-sharing Protein Production
-- Description of Risk-free Protein Production Project
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
-- Description of Protein Production Service in Insect Cells
- Protein Production Service in Yeast Expression System
-- Description of Protein Production Service in Yeast
- Protein Production Service in E. coli. Cells
-- Description of Protein Production Service in E. coli.
- Protein Refolding Service / Protein Production Service in E. coli.
-- Description of Protein Refolding & Production Project
- Poly His-tag Protein Production & Purification Service
-- Description of Risk-free Protein Production Project
-- Poly-his-tag Protein Expression, Production, and Purification Basics
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
-- DYKDDDK-tag (FLAG-tag) Protein Production Service Description
-- FLAG-tag Protein Expression, Production, and Purification Basics
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
-- Description of Fc-tag Protein Production & Purification Service
-- IgG-Fc-tag Protein Expression, Production, and Purification Basics
- HA-tag Protein Production & Purification Service
-- HA-tag Protein Production & Purification Service Description
-- HA-tag Protein Expression, Production, and Purification Basics
- Myc-tag Protein Production & Purification Service
-- Myc-tag Protein Production & Purification Service Description
-- Myc-tag Protein Expression, Production, and Purification Basics
- GST-fusion-tag Protein Production & Purification Service
-- GST-tag Protein Production & Purification Service Description
-- GST-tag Protein Expression, Production, and Purification Basics
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"