Goat Anti-Mouse IgG Secondary Antibody (HRP)

Cat: SSA007

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Overview
 

Description:

Goat polyclonal Secondary Antibody to Mouse IgG (HRP).

Host

Goat

Target species:

Mouse IgG

Specificity:

By ELISA this antibody reacts specifically with Mouse IgG.

 

Has cross-reactivity with rat IgG (>10%), human IgG (5.1%), rabbit IgG (2.9%), guinea pig IgG (2.7%)

Minimal cross-reactivity with bovine IgG, horse IgG

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Protected from prolonged exposure to light. Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt. Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purification notes:

Immunogen affinity purified

Formulation:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.5-10 μg/mL

ELISA

0.01-0.5 μg/mL

WB

0.05-1 μg/mL

(Colormetric: 0.1-1 μg/mL; Chemiluminescent: 0.05-1 μg/mL)

IP

0.05-1 μg/mL

 

Other Proteins

Expression of Programmed cell death protein 1 (PD-1) and Indoleamine 2,3-dioxygenase (IDO) in the tumor microenvironment and in tumor-draining lymph nodes of breast cancer

Author:
Ye, Q;Wang, C;Xian, J;Zhang, M;Cao, Y;Cao, Y;
Year:
2018
Journal:
Hum. Pathol.

PubMed ID: 29447919

 
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