Goat Anti-Rat IgG Secondary Antibody (Biotin)

 
 
Overview
 

Description:

Goat polyclonal Secondary Antibody to Rat IgG (Biotin).

Host

Goat

Target species:

Rat IgG

Specificity:

By ELISA this antibody reacts specifically with Rat IgG.

 

Has cross-reactivity with human IgG, mouse IgG, rabbit IgG (>10%), bovine IgG (2.5%), horse IgG (7.2%), guinea pig IgG (6.8%)

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Store at +4℃ short term (1-2 weeks). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt.Avoid repeated freeze-thaw cycles.

Constituents:

3% BSA, PBS, 0.1% Proclin300

Purity:

Immunogen affinity purified

Purification notes:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

 
Applications
 

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.2-5 μg/mL

ELISA

0.004-0.2 μg/mL

 WB

0.004-0.2 μg/mL

(Colormetric: 0.004-0.2 μg/mL; Chemiluminescent: 0.002-0.1 μg/mL)

IP

0.004-0.2 μg/mL

 
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