|Nature of sample||Choosing a secondary antibody when need|
|Species of sample||The antibodies for dual staining|
|Species of primary antibody host||Fluorochrome and chromogen labels|
|All Produced in House||8-156 Fold Improvement in ELISA Detection Limit|
|All Generated against Active Proteins or Precise Epitope||Majority>95% purity Some>99%|
|Second Generation of Rabbit Mabs||Over 1000 kinds of Flow Cytometry Antibodies|
|About 900 Rabbit / Mouse Mabs|
Even if the proteins of different species with the same name, they may be evolutionarily related, belong to the same protein family, but they may have different sequences and tertiary structures. One antibody may most likely not recognize the antigen which is come from different species to the target antigen. To ensure accurate results, you should choose an antibody that has been raised against the same species your sample is from. The antibody may react with the same target protein from other species sharing sufficient amino acid sequence homology.
If your sample is not from one of the species listed in the datasheet, this means that the species has not been tested and we cannot demonstrate suitability. A prediction of cross-reactivity is made based on sequence similarity.
The species the primary antibody is raised in should be different from the species of your sample. This is to avoid cross-reactivity of the secondary anti-immunoglobulin antibody with endogenous immunoglobulins in the sample.
For instance, if you are studying a mouse protein, choose a primary antibody that is raised in a different species. A primary antibody raised in rabbit will be an appropriate choice, followed by an anti-rabbit IgG secondary antibody conjugated to a detection molecule (enzyme, fluorochrome, biotin, etc.). This issue can be avoided if a conjugated primary antibody is used.
For techniques using samples that do not contain endogenous immunoglobulin (IgG), the choice of host species of the primary antibody is less critical. An example is western blotting of a cell lysate that is not expected to contain IgG. However, tissue lysates and tissue culture supernatants that contain serum will contain immunoglobulins. IgG will appear in western blots of reduced, denatured samples as bands at 50 and 25 kDa corresponding to the heavy and light chains of the IgG molecule.