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Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service

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Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
Fc Tag Design: Fusion with protein at N- or C-terminus
Choices of Fc-tags: Human IgG1-Fc, IgG2-Fc, IgG3-Fc, IgG4-Fc
Mouse IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc
Rat IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG2c-Fc
Rabbit IgG-Fc
Canine IgG-Fc
Cleavage Site: Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
Purification: Affinity Purification by: Protein A Affinity Resin,
Protein G Affinity Resin, or Protein L Affinity Resin
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 8-12 weeks

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas

Fc-Fusion-tag Protein Expression, Production, and Purification Basics

What is an IgG-Fc-tag or IgG-Fc Chimeric?

IgG-Fc tag is the constant region (domain 3 and 4) of immunoglobulin heavy-chain. It is fused to the C-terminus of a protein and hence it recembles a mouse/human chimeric antibody in a way, and sometimes the Fc-fusion protein is also called Fc chimeric protein. The Fc-tag is about 25 KDa.

Why use an IgG-Fc-tag for protein expression and production?

Adding an IgG-Fc tag to a protein not only allows rapid and simple...... < Learn More>

Fc-fusion-tag (Fc-chimeric) Protein Production & Purification Service Description

IgG-Fc-fusion-tag (Fc-chimeric protein) has been widely used in recombinant protein expression and production. The Fc-fusion-tag allows simple and efficient affinity purification of the tagged protein using protein A or protein G affinity chromatography. The Fc-fusion-tag (Fc-chimeric) also can be used for detection in western blot, ELISA assay by using commercially available antibodies and ELISA kits. The antibody Fc-region is typically fused to the C-terminus of protein and it is mostly used in mammalian cell expression system. The addition of the IgG-Fc could also increase protein solubility and expression yield sometimes. Due to the nature of the antibody IgG-Fc, Fc-chimeric proteins ...... < Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
-- Custom Protein Production Project Description
-- Description of Risk-sharing Protein Production
-- Description of Risk-free Protein Production Project
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
-- Description of Protein Production Service in Insect Cells
- Protein Production Service in Yeast Expression System
-- Description of Protein Production Service in Yeast
- Protein Production Service in E. coli. Cells
-- Description of Protein Production Service in E. coli.
- Protein Refolding Service / Protein Production Service in E. coli.
-- Description of Protein Refolding & Production Project
- Poly His-tag Protein Production & Purification Service
-- Description of Risk-free Protein Production Project
-- Poly-his-tag Protein Expression, Production, and Purification Basics
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
-- DYKDDDK-tag (FLAG-tag) Protein Production Service Description
-- FLAG-tag Protein Expression, Production, and Purification Basics
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
-- Description of Fc-tag Protein Production & Purification Service
-- IgG-Fc-tag Protein Expression, Production, and Purification Basics
- HA-tag Protein Production & Purification Service
-- HA-tag Protein Production & Purification Service Description
-- HA-tag Protein Expression, Production, and Purification Basics
- Myc-tag Protein Production & Purification Service
-- Myc-tag Protein Production & Purification Service Description
-- Myc-tag Protein Expression, Production, and Purification Basics
- GST-fusion-tag Protein Production & Purification Service
-- GST-tag Protein Production & Purification Service Description
-- GST-tag Protein Expression, Production, and Purification Basics
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"