ELISA Kit technology center

Sinobiological Inc. provide 100+ ELISA Kits for scientific research. Immunoassays are available for measuring a range of molecules including virus, cytokines, interleukin, chemokines, growth factors, CD molecule and more.

Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate, see in detail in the section of ELISA device) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. The part of antibody incubation of ELISA is similar with that of western blot. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

ELISA Antibody
- ELISA Kit features
ELISA Kit features-Comprehensive quality assurance
90% of The Kits are McAb-McAb Matching
Influenza Virus ELISA Kit Covering the General Strain
Standard Substance are Produced by Eukaryotic expressing system
Mouse Protein ELISA Kit
- ELISA Kit product center
human Taget
others species
ELISA Kit Cytokine
Stem Cell Research
Cancer Research
CD Molecule
Signal Transduction
Inflammation / Inflammatory Mediator
- ELISA Kit technology center
ELISA kit Introduction
ELISA Kit principle
ELISA kit protocol
ELISA kit troubleshooting
ELISA kit Tips
ELISA kit storage
ELISA kit material provided
ELISA Kit precaution and safety instructions
ELISA Kit Terms
ELISA Pair Set