ELISA Introduction

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries, such as ELISA application in food industry. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

ELISA Antibody
ELISA antibody features
ELISA Product center
ELISA technology center+
- ELISA Introduction
What is ELISA Mechanism?
What is being tested using ELISA assay?
ELISA Advantages
ELISA History
- ELISA Types
Indirect ELISA
Direct ELISA
Sandwich ELISA
Competitive ELISA
- ELISA Principle
Direct ELISA Principle
indirect ELSIA principle
Competitive ELISA principle
Sandwich ELISA principle
- ELISA Protocol
Indirect ELISA Protocol
Direct ELISA Protocol
Sandwich ELISA Protocol
Competitive ELISA Protocol
- ELISA Terms
- ELISA Tips
on Washing
on Pipetting
on Microplate
on Temperature
on Incubation
on Others
- ELISA Troubleshooting
High Background
Low OD Readings
No Color Development
Poor Reproducibility
- ELISA Device
- ELISA Detection Strategies
Chromogenic Assay
Chemifluorescent Assay
Chemiluminescent Assay