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ELISA Antibody

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• Use High Quality Recombinant Proteins • 4000+ ELISA Antibodies
• Sufficient Cross-Reactivity Data • Strict Quality Control
• Covering Multiple Species • High Sensitivity
-----Human, Mouse,Rat,Monkey,Virus,Ferret,etc. • Low Background
  • Rabbit Mab, Mouse Mab, Rabbit Pab
Full list of ELISA Antibody  
  • Provide for:
  • • 4000+ ELISA antibodies
  • • more than 20 species
  • • High Sensitivity
  • • Low Background
  • • Affordable

By Molecue:

• EGFR • PDCD1
• VEGFA • IL6
• IL12A • CST6
• IL1B • HGF
• LEP • TNF
• IL15 • CD38

By Species :

• Human • Ferret
• Mouse • Rhesus
• Rat • E.coli
• Cynomolgus • Aequorea victoria
• Canine • Virus
• Rabbit • Other species

By Antibody Type :

• Mouse Monoclonal Antibody
• Rabbit Monoclonal Antibody
• Rabbit Polyclonal Antibody

By Recearch Area:

• CD Molecule • Receptor
• Biomarker • Cytokine
• Biological • Interkeukin
• Diagnostic • Cancer Research
• Stem Cell Research

ELISA Introduction

ELISA Types

ELISA Principle

ELISA Protocol

ELISA Terms

ELISA Tips

ELISA Troubleshooting

ELISA FAQ

ELISA Device

ELISA Detection Strategies

ELISA Antibody Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries,such as ELISA application in food industry. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate, see in detail in the section of ELISA device) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. The part of antibody incubation of ELISA is similar with that of western blot. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"