Mouse eIF2AK1/HRI Gene cDNA Clone / ORF Clone

eIF2AK1/HRI cDNA Clone Datasheet

eIF2AK1/HRI cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013557.2
• cDNA Size: 1860
• Gene Synonym: Annotation category: partial on reference assembly
• Source: Mouse

eIF2AK1/HRI cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus eukaryotic translation initiation factor 2 alpha kinase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

eIF2AK1/HRI cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

eIF2AK1/HRI cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

eIF2AK1/HRI cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGGGGG GCAGCTCCGT GGACGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG
61       GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG
121      TCCGATGTCC CCGCAGAGCT CCAAGTGTTA AAAGAGCCCC TACAGCAGCC CACGTTCCCT
181      TTCCTGGTGG CAAACCAGCT GCTGCTCGTC TCCTTACTGG AACACTTGAG CCATGTGCAC
241      GAGCCAAACC CGCTCCACTC CAAACAGGTG TTTAAATTAC TGTGCCAGAC TTTTATCAAG
301      ATGGGGCTGC TCTCTTCCTT TACCTGCAGT GATGAGTTTA GCTCTCTGAG ACTCCACCAC
361      AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAAGAGA GAGTCCGTCA GGATCCTTGT
421      CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGAGAGA TAGCCTTCGA AGCACAAACG
481      TCACGCTACT TAAATGAATT TGAAGAGCTT GCCATCTTAG GGAAAGGAGG ATATGGAAGA
541      GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT
601      AAGAGCGCAA CTAAAACAGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGT
661      CTCCAGCATC CCAATATTGT TGGCTACCAC ACTGCGTGGA TAGAACATGT TCATGTGGTT
721      CAGCCACAAG ACAGAGTTCC GATTCAACTG CCCTCTCTCG AAGTGCTGTC GGAGCAGGAA
781      GGGGACAGAG ACCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TGTCTTTGCT
841      GAACTCACCC CAGAAAAAGA AAAACCTTTT GGGGAGTCTG AGGTTAAAAA TGAGAATAAC
901      AACCTGGTGA GCTACACGGC CAACTTAGTG GTCAGGAACA GCAGTGAAAG TGAATCGTCC
961      ATTGAGCTCC AAGAAGACGG CTTGACTGAT TTGTCCGTCA GACCAGTTGT CAGGCATCAG
1021     CTGCCGCTGG GGCATAGCTC GGAATTGGAA GGGAATTTTA CATCCACGGA TGAGTCTTCT
1081     GAAGGCAACT TGAACCTGCT GGGGCAGACG GAGGTTCGGT ACCACCTGAT GTTGCACATC
1141     CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAA CTGAGAGGAA CAAGCGGAGC
1201     CGGGAGTATG TGGACGAAGC TGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAAATT
1261     TTTCAAGAAT TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATTGT CCACAGAGAT
1321     CTGAAGCCTA GAAATATTTT TCTTCATGGC CCTGATCAGC AAGTAAAAAT AGGAGACTTT
1381     GGTCTGGCCT GTGCAGACAT CATCCAGAAT GCAGACTGGA CCAACAGAAA TGGGAAAGGA
1441     ACACGGACAC ACACATCCAG AGTGGGGACT TGTCTCTACG CGTCACCGGA ACAGCTGGAG
1501     GGATCCCAGT ACGATGCCAA GTCAGATATG TATAGCTTGG GTGTGATCCT GCTCGAGCTC
1561     TTTCAGCCAT TCGGGACAGA AATGGAGAGG GCAACAGTCT TAACAGGCGT AAGGACTGGT
1621     CGGATACCCG AATCCCTCAG TAAAAGGTGT CCGGTGCAAG CCAAGTATAT CCAGCTCCTA
1681     ACCGGGAGGA ATGCGTCACA GAGACCATCT GCCCTTCAGC TGCTGCAGAG TGAGCTTTTT
1741     CAAACAACTG GAAATGTTAA TCTCACATTG CAGATGAAGA TAATAGAACA AGAGAAGGAA
1801     ATTGAAGAAC TAAAGAAGCA ACTAAGCCTC CTTTCTCAGG ACAGAGGGCT GAAGAGATGA

 

Quality Control:cDNA sequencing results is available upon request.