Anti-E-Cadherin Antibody

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Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-E-Cadherin Antibody
Validated applications
WB,ELISA,IHC-P,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human E-Cadherin
Immunogen
Recombinant Human E-Cadherin / CDH1 / E-cad / CD324 protein (Catalog#10204-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human E-Cadherin / CDH1 / E-cad / CD324 (rh E-Cadherin / CDH1 / E-cad / CD324; Catalog#10204-H08H; NP_004351.1; Met1-Ile707). E-Cadherin / CDH1 / E-cad / CD324 specific IgG was purified by Human E-Cadherin / CDH1 / E-cad / CD324 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
ICC-IF 1:50-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human E-cadherin in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human E-cadherin in human gastric cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human E-cadherin in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-E-CAD rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:97 kDa

Observed band size:120 kDa

(We are unsure as to the identity of these extra bands.)

Immunofluorescence staining of Human E-CAD in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human E-CAD polyclonal antibody (1:200). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membrane.

Anti-E-Cadherin Antibody: Alternative Names

Anti-Arc-1 Antibody; Anti-CD324 Antibody; Anti-CDH1 Antibody; Anti-CDHE Antibody; Anti-E-cad Antibody; Anti-E-Cadherin Antibody; Anti-ECAD Antibody; Anti-LCAM Antibody; Anti-UVO Antibody

E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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