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DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service

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DYKDDDK-tag (FLAG-Tag) Protein Production & Purification Service

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
FLAG Tag Design: DYKDDDDK-tag (FLAG-tag) at N- or C-terminus
Cleavage Site: Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
Purification: Affinity Purification by FLAG-tag Antibody Affinity Resin
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 8-12 weeks

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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FLAG-tag Protein Expression, Production, and Purification Basics

What is a FLAG-tag?

A FLAG-tag, or FLAG octapeptide, is a peptide sequence of DYKDDDDK, which can be fused tgo either the N- or C-terminus of a protein to facilitate detection and purification by a FLAG-tag specific monoclonal antibody.

Why use a FLAG-tag for protein expression and production?

Adding a FLAG-tag to the N- or C-terminus of a protein allows rapid purification of the over-expressed recombinant protein, by using a FLAG-tag specific monoclonal antibody conjugated to agarose beads. Previsouly, commmercial FLAG-tag affinity purification resin is very ...... < Learn More>

DYKDDDK-tag (FLAG-tag) Protein Production & Purification Service Description

DYKDDDK-tag (FLAG-tag) has been widely used in recombinant protein expression and production. The FLAG-tag allows simple and efficient affinity purification of the tagged protein using FLAG-tag specific antibody conjugated to agarose-beads. The FLAG-tag (DYKDDDK-tag) also can be used for detection in western blot by using a FLAG-tag-specific antibody. FLAG-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.

Sino Biological has successfully produced many proteins with a FLAG-tag and purified with FLAG-affinity chromatography. We are highly experienced in designing the FLAG-tag according to ...... < Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
- Protein Prodution Service description
- Protein protuction Capacity decription
- Description of Protein Production Experience and Track Records
- Protein production technology platform description
- Custom Protein Production project-service model
-- Custom Protein Production Project Description
-- Description of Risk-sharing Protein Production
-- Description of Risk-free Protein Production Project
- transient transfection protein production service
- Protein production service in CHO cell culture stable production
- Protein Production Service in Insect Cell /Baculovirus Expression
-- Description of Protein Production Service in Insect Cells
- Protein Production Service in Yeast Expression System
-- Description of Protein Production Service in Yeast
- Protein Production Service in E. coli. Cells
-- Description of Protein Production Service in E. coli.
- Protein Refolding Service / Protein Production Service in E. coli.
-- Description of Protein Refolding & Production Project
- Poly His-tag Protein Production & Purification Service
-- Description of Risk-free Protein Production Project
-- Poly-his-tag Protein Expression, Production, and Purification Basics
- DYKDDDDK-Tag (FLAG-Tag) Protein Production & Purification Service
-- DYKDDDK-tag (FLAG-tag) Protein Production Service Description
-- FLAG-tag Protein Expression, Production, and Purification Basics
- Fc-fusion-tag / Fc-Chimeric Protein Production & Purification Service
-- Description of Fc-tag Protein Production & Purification Service
-- IgG-Fc-tag Protein Expression, Production, and Purification Basics
- HA-tag Protein Production & Purification Service
-- HA-tag Protein Production & Purification Service Description
-- HA-tag Protein Expression, Production, and Purification Basics
- Myc-tag Protein Production & Purification Service
-- Myc-tag Protein Production & Purification Service Description
-- Myc-tag Protein Expression, Production, and Purification Basics
- GST-fusion-tag Protein Production & Purification Service
-- GST-tag Protein Production & Purification Service Description
-- GST-tag Protein Expression, Production, and Purification Basics
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"