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Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone)

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RSV-FcDNA Clone Product Information
Gene_bank_ref_id:
cDNA Size:1725
cDNA Description:ORF Clone of Human RSV (subtype A, strain Long) Fusion glycoprotein / RSV-F DNA.
Gene Synonym:F, HRSVgp08
Species:RSV
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with P12568.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone) on other vectors
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-GFPSpark-taggedVG40039-ACG$345
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-OFPSpark tagVG40039-ACR$345
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-FLAG-taggedVG40039-CF$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-His-taggedVG40039-CH$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-Myc-taggedVG40039-CM$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, C-HA-taggedVG40039-CY$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, FLAG-taggedVG40039-G-F$395
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-FLAG-taggedVG40039-NF$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-His-taggedVG40039-NH$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-Myc-taggedVG40039-NM$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-HA-taggedVG40039-NY$315
Human respiratory syncytial virus (RSV) (subtype A, strain Long) Fusion glycoprotein / RSV-F Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, untaggedVG40039-UT$315
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Background

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F0), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

References
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
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    Catalog:VG40039-G
    List Price: $195.00  (Save $80.00)
    Price:$115.00      [How to order]
    Availability5 business days
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