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Influenza A H16N3 (A/black-headed gull/Sweden/5/99) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)

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H16N3 HA cDNA Clone Product Information
Gene_bank_ref_id:
RefSeq ORF Size:1701bp
cDNA Description:Full length Clone DNA of Influenza A H16N3 (A/black-headed gull/Sweden/5/99) HA.
Gene Synonym:HA1, Hemagglutinin
Species:H16N3
Vector:Vector 3
Plasmid:pCDNA3-H16N3-BS-99-HA
Restriction Site:HindIII + XbaI (5.4kb + 1.7kb)
Tag Sequence:
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AAV91217.1.
Sequencing primers:Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAA
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
Vector 3 Information

The Vector 3 is 5.4kb in length, and contains the the amplicin resistance gene, conferring selection of the plasmid in E. coli., the kanamycin resistance gene (neomycin for mammalian selection), and the CMV promoter upstream of the cDNA insert. The SV40 origin allows for replication in mammalian cells, the ColE1 origin is the bacterial origin of replication, and the f1 origin is the filamentous phage origin of replication. The plasmid has multiple cloning sites as shown below. NOTE: the restriction enzymes used in each vector 3 clone is indicated in the product datasheet.

Vector 3 Usage Suggestion

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with primer pair SP6 and T7.

Vector Sequence Download
Influenza A H16N3 (A/black-headed gull/Sweden/5/99) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized) on other vectors
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