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Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set

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Influenza HA Materials provided
Capture Ab:0.5 mg/mL of rabbit anti-H1N1 ( A/Puerto Rico/8/1934 ) HA monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.5 mg/mL rabbit anti-H1N1 ( A/Puerto Rico/8/1934 ) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 50 ng of recombinant H1N1 (A/Puerto Rico/8/1934) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
Streptavidin-HRP:
Influenza HA Specificity
Influenza HA Sensitivity
The minimum detectable dose of H1N1 (A/Puerto Rico/8/1934) HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Influenza HA Principle of the product
The H1N1 (A/Puerto Rico/8/1934) HA ELISA Pair Set is for the quantitative determination of H1N1 (A/Puerto Rico/8/1934) HA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H1N1 ( A/Puerto Rico/8/1934 ) HA coated on a 96-well plate. Standards and samples are added to the wells, and any H1N1 ( A/Puerto Rico/8/1934 ) HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-H1N1 ( A/Puerto Rico/8/1934 ) HA monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H1N1 ( A/Puerto Rico/8/1934 ) HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Influenza HA Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
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Catalog: SEKA11684-5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"