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Cynomolgus CD32a / Fc gamma RIIA / FCGR2A ELISA Pair Set

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Cynomolgus CD32a Materials provided
Capture Ab:0.5 mg/mL of mouse anti-Cynomolgus FCGR2A monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.25 mg/mL mouse anti-Cynomolgus FCGR2A monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 18 ng of recombinant Cynomolgus FCGR2A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
Streptavidin-HRP:
Cynomolgus CD32a Specificity
Cynomolgus CD32a Sensitivity
The minimum detectable dose of Cynomolgus CD32a / Fc gamma RIIA / FCGR2A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Cynomolgus CD32a Principle of the product
The Cynomolgus CD32a / Fc gamma RIIA / FCGR2A ELISA Pair Set is for the quantitative determination of Cynomolgus CD32a / Fc gamma RIIA / FCGR2A.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32a / Fc gamma RIIA / FCGR2A coated on a 96-well plate. Standards and samples are added to the wells, and any CD32a / Fc gamma RIIA / FCGR2A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD32a / Fc gamma RIIA / FCGR2A monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD32a / Fc gamma RIIA / FCGR2A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Cynomolgus CD32a Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Cynomolgus CD32a ELISA Pair Set Standard Curve
Cynomolgus CD32a / Fc gamma RIIA / FCGR2A ELISA standard curve
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Catalog: SEK90016-5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"