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Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set

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Influenza HA Materials provided
Capture Ab:1 mg/mL of rabbit anti-Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 0.5 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL of rabbit anti-Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 110 ng of recombinant Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Streptavidin-HRP:
Influenza HA Specificity
Influenza HA Sensitivity
The minimum detectable dose of Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Influenza HA Principle of the product
The Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set is for the quantitative determination of Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA coated on a 96-well plate. Standards and samples are added to the wells, and any Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Influenza HA Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
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Catalog: SEK40103-5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"