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Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin / HA ELISA Pair Set

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Influenza HA Materials provided
Capture Ab:0.5 mg/mL of mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.4 mg/mL mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 140 ng of recombinant H4N6 (A/mallard/Ohio/657/2002) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 7 ng/mL is recommended.
Influenza HA Specificity
Influenza HA Sensitivity
The minimum detectable dose of H4N6 (A/mallard/Ohio/657/2002) HA was determined to be approximately 109.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Influenza HA Principle of the product
The H4N6 (A/mallard/Ohio/657/2002) HA ELISA Pair Set is for the quantitative determination of H4N6 (A/mallard/Ohio/657/2002) HA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H4N6 (A/mallard/Ohio/657/2002) HA coated on a 96-well plate. Standards and samples are added to the wells, and any H4N6 (A/mallard/Ohio/657/2002) HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H4N6 (A/mallard/Ohio/657/2002) HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Influenza HA Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
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Catalog: SEK11714-5
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