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Human METTL1 ELISA Pair Set

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Materials provided
Capture Ab:0.25 mg/mL of mouse antiMETTL1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating
Detection Ab:0.5 mg/mL mouse anti-METTL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use
Standard:Each vial contains 100 ng of recombinant METTL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
The minimum detectable dose of human METTL1 was determined to be approximately 156.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the product
The human METTL1 ELISA Pair Set is for the quantitative determination of human METTL1 .
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for METTL1coated on a 96-well plate. Standards and samples are added to the wells, andany METTL1 present binds to the immobilized antibody. The wells are washed anda horseradish peroxidase conjugated mouse anti-METTL1 monoclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of METTL1 present in the sample. To end the enzymereaction, the stop solution is added and absorbances of the microwell are readat 450 nm.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

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