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Influenza A H9N2 Hemagglutinin / HA ELISA Pair Set

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Materials provided
Capture Ab:0.5 mg/mL of mouse anti-H9N2 HA monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
Detection Ab:1.0 mg/mL rabbit anti-H9N2 HA polyclonal antibody conjugated to horseradish -peroxidase (HRP). Dilute to a working concentration of 0.4 μg/mL in detection antibody dilution buffer before use
Standard:Each vial contains 160 ng of recombinant H9N2 HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended
The minimum detectable dose of Influenza A H9N2 HA was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the product
The H9N2 HA ELISA Pair Set is for the quantitative determination of Influenza A H9N2 HA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H9N2 HAcoated on a 96-well plate. Standards and samples are added to the wells, andany H9N2 HA present binds to the immobilized antibody. The wells are washed anda horseradish peroxidase conjugated rabbit anti-H9N2 HA polyclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of H9N2 HA present in the sample. To end the enzymereaction, the stop solution is added and absorbances of the microwell are readat 450 nm.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
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