|Datasheet||Specific References||Reviews||Related Products||Protocols|
|0.5 mg/mL of mouse anti-HPX monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.|
|0.4 mg/mL rabbit anti-HPX polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.|
|Each vial contains 25 ng of recombinant HPX. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.|
|The minimum detectable dose of human HPX / Hemopexin was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
The human HPX / Hemopexin ELISA Pair Set is for the quantitative determinationof human HPX / Hemopexin.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for HPX / Hemopexin coated on a 96-well plate. Standards and samples are added tothe wells, and any HPX / Hemopexin present binds to the immobilizedantibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-HPX polyclonalantibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of HPX / Hemopexin present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
|Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.|
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.