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Human Hemopexin / HPX ELISA Pair Set

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Human HPX Materials provided
Capture Ab:0.5 mg/mL of mouse anti-HPX monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.4 mg/mL rabbit anti-HPX polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 25 ng of recombinant HPX. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
Human HPX Specificity
Human HPX Sensitivity
The minimum detectable dose of human HPX / Hemopexin was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human HPX Principle of the product
The human HPX / Hemopexin ELISA Pair Set is for the quantitative determinationof human HPX / Hemopexin.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for HPX / Hemopexin coated on a 96-well plate. Standards and samples are added tothe wells, and any HPX / Hemopexin present binds to the immobilizedantibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-HPX polyclonalantibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of HPX / Hemopexin present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
Human HPX Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human HPX ELISA Pair Set Standard Curve
Human Hemopexin / HPX ELISA standard curve
Product nameProduct name
  • Human Hemopexin / HPX ELISA standard curve