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Human CEACAM6 / CD66c ELISA Pair Set

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Human CEACAM6 Materials provided
Capture Ab:1 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.4 mg/mL of biotinylated rabbit anti-CEACAM6 polyclonal antibody. Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 88 ng of recombinant CEACAM6. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Streptavidin-HRP:50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Human CEACAM6 Specificity
Human CEACAM6 Sensitivity
The minimum detectable dose of human CEACAM6 / CD66c was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CEACAM6 Principle of the product
The human CEACAM6 / CD66c ELISA Pair Set is for the quantitative determination of human CEACAM6 / CD66c.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CEACAM6 / CD66c coated on a 96-well plate. Standards and samples are added to the wells, and any CEACAM6 / CD66c present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-CEACAM6 / CD66c polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of CEACAM6 / CD66c present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.
Human CEACAM6 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Human CEACAM6 ELISA Pair Set Standard Curve
Human CEACAM6 / CD66c ELISA Pair Set
CEACAM6/CD66c Background

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

Human CEACAM6/CD66c References
  • Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.
  • Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.
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    • Human CEACAM6 / CD66c ELISA Pair Set