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Human VEGFR3 / FLT4 ELISA Pair Set

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Human VEGFR3/FLT-4 Materials provided
Capture Ab:0.5 mg/mL of mouse anti-VEGFR3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating
Detection Ab:0.4 mg/mL mouse anti-VEGFR3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use
Standard:Each vial contains 80 ng of recombinant VEGFR3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended
Streptavidin-HRP:
Human VEGFR3/FLT-4 Specificity
Human VEGFR3/FLT-4 Sensitivity
The minimum detectable dose of human VEGFR3 / FLT4 was determined to be approximately 156.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human VEGFR3/FLT-4 Principle of the product
The human VEGFR3 / FLT4 ELISA Pair Set is for the quantitative determination of human VEGFR3 / FLT4.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for VEGFR3 /FLT4 coated on a 96-well plate. Standards and samples are added to the wells,and any VEGFR3 / FLT4 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated mouse anti-VEGFR3 / FLT4monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of VEGFR3 / FLT4 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
Human VEGFR3/FLT-4 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human VEGFR3/FLT-4 ELISA Pair Set Standard Curve
Human VEGFR3 / FLT4 ELISA Pair Set
VEGFR3/FLT-4  Background

Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.

Human VEGFR3/FLT-4  References
  • Shushanov S, et al. (2000)VEGFc and VEGFR3 expression in human thyroid pathologies. Int J Cancer.86(1): 47-52.
  • Iljin K, et al. (2001) VEGFR3 gene structure, regulatory region, and sequence polymorphisms. FASEB J. 15(6): 1028-36.
  • Liu XE, et al. (2004) Expression and significance of VEGF-C and FLT-4 in gastric cancer. World J Gastroenterol. 10(3): 352-5.
  • Stearns ME, et al. (2004) Expression of a flt-4 (VEGFR3) splicing variant in primary human prostate tumors. VEGF D and flt-4t(Delta773-1081) overexpression is diagnostic for sentinel lymph node metastasis. Lab Invest. 84(6): 785-95.
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