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Human CD48 / SLAMF2 / BCM1 ELISA Pair Set

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Human CD48 Materials provided
Capture Ab:0.4 mg/mL of mouse anti-CD48 monoclonal antibody. Dilute to a working concentration of 0.5 μg/mL in CBS before coating.
Detection Ab:1.0 mg/mL of rabbit anti-CD48 polyclonal antibody. Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 20 ng of recombinant CD48. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Streptavidin-HRP:50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Human CD48 Specificity
Human CD48 Sensitivity
The minimum detectable dose of human CD48 (BCM-1 / SLAMF2) was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CD48 Principle of the product
The human CD48 (BCM-1 / SLAMF2) ELISA Pair Set is for the quantitative determination of human CD48.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD48 ( BCM-1 / SLAMF2) coated on a 96-well plate. Standards and samples are added tothe wells, and any CD48 (BCM-1 / SLAMF2) present binds to the immobilizedantibody. The wells are washed and a biotinylated rabbit anti-CD48 polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Toproduces color in proportion to the amount of CD48 (BCM-1 / SLAMF2) presentin the sample strepavidin-HRP and TMB substrate solution are loaded. Theabsorbances of the microwell are read at 450 nm.
Human CD48 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Human CD48 ELISA Pair Set Standard Curve
Human CD48 / SLAMF2 / BCM1 ELISA Pair Set
CD48/SLAMF2 Background

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Human CD48/SLAMF2 References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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    Catalog: SEK10797-5
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"