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Human Neuropilin-2 / NRP2 ELISA Pair Set

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Human NRP2 Materials provided
Capture Ab:0.25 mg/mL of mouse anti-NRP2 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
Detection Ab:0.5 mg/mL mouse anti-NRP2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 200 ng of recombinant NRP2. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
Streptavidin-HRP:
Human NRP2 Specificity
Human NRP2 Sensitivity
The minimum detectable dose of Human Neuropilin-2 / NRP2 was determined to be approximately 156.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human NRP2 Principle of the product
The human Neuropilin-2 / NRP2 ELISA Pair Set is for the quantitative determinationof human Neuropilin-2 / NRP2.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Neuropilin-2 / NRP2 coated on a 96-well plate. Standards and samples are added to the wells, and any Neuropilin-2 / NRP2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Neuropilin-2 / NRP2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Neuropilin-2 / NRP2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human NRP2 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Neuropilin-2/NRP2 Background

Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classⅢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as formation of small lymphatic vessels and capillaries. Recent study has identified NRP-2 as a polysialylated protein expressed in human dendritic cells and modulates DC-T cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis and metastasis.

Human Neuropilin-2/NRP2 References
  • Chen H. et al., 1997, Neuron. 19: 547-59.
  • Bielenberg DR. et al., 2006, Exp Cell Res. 312: 584-93.
  • Yuan L. et al., 2002, Development. 129: 4797-806.
  • Benoit F. et al., 2006, Blood. 108: 1243-50.
  • Sabrina C. et al., 2007, J Biol Chem. 282: 30346-56.
  • Gray MJ. et al., 2008, J Natl Cancer Inst. 100: 109-20.
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