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Human EpCAM / TROP-1 / TACSTD1 ELISA Pair Set

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Human EpCAM Materials provided
Capture Ab:0.2 mg/mL of mouse anti-EpCAM monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating
Detection Ab:0.5 mg/mL of rabbit anti-EpCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use
Standard:Each vial contains 50 ng of recombinant EpCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended
Human EpCAM Specificity
Human EpCAM Sensitivity
The minimum detectable dose of human EpCAM / TROP1 was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human EpCAM Principle of the product
The human EpCAM / TROP1 ELISA Pair Set is for the quantitative determinationof human EpCAM / TROP1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for EpCAM / TROP1 coated on a 96-well plate. Standardsand samples are added to the wells, and any EpCAM / TROP1 presentbinds to the immobilized antibody. The wells are washed and a biotinylatedrabbit anti-EpCAM monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". To produces color in proportion to theamount of EpCAM / TROP1 presentin the sample strepavidin-HRP and TMB substrate solution are loaded. Theabsorbances of the microwell are read at 450 nm.
Human EpCAM Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human EpCAM ELISA Pair Set Standard Curve
Human EpCAM / TROP-1 / TACSTD1 ELISA standard curve
EpCAM/TROP-1/TACSTD1 Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Human EpCAM/TROP-1/TACSTD1 References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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