|Datasheet||Specific References||Reviews||Related Products||Protocols|
|0.5 mg/mL of mouse anti-IL6R monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.|
|Each vial contains 120 μg biotinylated rabbit anti-IL6R polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.|
|Each vial contains 16 ng of recombinant IL6R. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.|
|50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.|
|The minimum detectable dose of human IL6R / CD126 was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
The human IL6R / CD126 ELISA Pair Set is for the quantitative determination of human IL6R / CD126
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL6R /CD126 coated on a 96-well plate. Standards and samples are added to the wells,and any IL6R / CD126 present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-IL6R / CD126 polyclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of IL6R / CD126 present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.
|Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.|
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.