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Human PD1 / PDCD1 / CD279 ELISA Pair Set

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Human PD1/PDCD1 Materials provided
Capture Ab:0.5 mg/mL of mouse anti-PD1 monoclonal antibody. Dilute to a working concentration of 4 μg/mL in CBS before coating.
Detection Ab:Each vial contains 120 μg biotinylated rabbit anti-PD1 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 25 ng of recombinant PD1. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
Streptavidin-HRP:50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Human PD1/PDCD1 Specificity
Human PD1/PDCD1 Sensitivity
The minimum detectable dose of human PD1 / PDCD1 was determined to be approximately 39.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human PD1/PDCD1 Principle of the product
The human PD1 / PDCD1 ELISA Pair Set is for the quantitative determination of human PD1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for PD1 /PDCD1 coated on a 96-well plate. Standards and samples are added to the wells,and any PD1 / PDCD1 present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-PD1 polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of PD1 / PDCD1 present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.
Human PD1/PDCD1 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
PD1/PDCD1/CD279 Background

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Human PD1/PDCD1/CD279 References
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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    • Human PD1 / PDCD1 / CD279 ELISA standard curve