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Human IL18BP / IL18BPa ELISA Pair Set

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Human IL18BP Materials provided
Capture Ab:0.5 mg/mL of mouse anti-IL18BPa monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:Each vial contains 120 μg biotinylated rabbit anti-IL18BPa polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 42 ng of recombinant IL18BPa. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
Streptavidin-HRP:50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Human IL18BP Specificity
Human IL18BP Sensitivity
The minimum detectable dose of human IL18BP / IL18BPa was determined to be approximately 23.4 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human IL18BP Principle of the product
The human IL18BP / IL18BPa ELISA Pair Set is for the quantitative determination of human IL18BPa.
This ELISA Pair Set contains the basic components required for the development ofsandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL18BP /IL18BPa coated on a 96-well plate. Standards and samples are added to thewells, and any IL18BP / IL18BPa present binds to the immobilized antibody. Thewells are washed and a biotinylated rabbit anti-IL18BPa polyclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". To producescolor in proportion to the amount of IL18BP / IL18BPa present in the samplestrepavidin-HRP and TMB substrate solution are loaded. The absorbances of themicrowell are read at 450 nm.
Human IL18BP Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Human IL18BP ELISA Pair Set Standard Curve
Human IL18BP / IL18BPa ELISA Pair Set
IL18BP / IL18BPa Background

Interleukin-18-binding protein (IL-18BP) is a constitutively expressed and secreted protein. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. The adjacently located family members IL18 Receptor 1 (IL18R1) and IL18 receptor accessory protein (IL18RAP) may also be important in the development of asthma and atopy. IL-18 binding protein (IL-18BP) was only moderately elevated, resulting in a high level of biologically active free IL-18 in HPS. A severe IL-18/IL-18BP imbalance results in Th-1 lymphocyte and macrophage activation, which escapes control by NK-cell cytotoxicity and may allow for secondary HPS in patients with underlying diseases.

Human IL18BP / IL18BPa References
  • Novick D, et al.. (2001) A novel IL-18BP ELISA shows elevated serum IL-18BP in sepsis and extensive decrease of free IL-18. Cytokine. 14(6): 334-42.
  • Mazodier K, et al.. (2005) Severe imbalance of IL-18/IL-18BP in patients with secondary hemophagocytic syndrome. Blood. 106(10): 3483-9.
  • Akira S. (2000) The role of IL-18 in innate immunity. Curr Opin Immunol. 12(1): 59-63.
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