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Human CD32b / FCGR2B ELISA Pair Set

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Materials provided
Capture Ab:0.5 mg/mL of mouse anti-CD32b monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:0.6 mg/mL of biotinylated rabbit anti-CD32b polyclonal antibody. Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 15 ng of recombinant CD32b. Reconstitute with 1 mL detection antibody dilution buffer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
Sensitivity
The minimum detectable dose of human CD32b (Fc-gamma-RIIb / FCGR2B) was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the product
The human CD32b (Fc-gamma-RIIb / FCGR2B) ELISA Pair Set is for the quantitative determination of human CD32b.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32b (Fc-gamma-RIIb / FCGR2B) coated on a 96-well plate. Standards and samples are added to the wells, and any CD32b present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-CD32b polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of CD32b present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
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Catalog:SEK10259-5
List Price: $350.00  (Save $0.00)
Price:$350.00      [How to order]
AvailabilityIn Stock
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  • Human CD32b / FCGR2B ELISA standard curve
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