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Human DKK3 / Dkk-3 / REIC ELISA Pair Set

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Human DKK3 Materials provided
Capture Ab:0.5 mg/mL of mouse anti-DKK3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab:Each vial contains 120 μg biotinylated rabbit anti-DKK3 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 98 ng of recombinant DKK3. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Streptavidin-HRP:50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Human DKK3 Specificity
Human DKK3 Sensitivity
The minimum detectable dose of human Dkk3 / REIC was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human DKK3 Principle of the product
The human Dkk3 / REIC ELISA Pair Set is for the quantitative determination of human Dkk3.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Dkk3 /REIC coated on a 96-well plate. Standards and samples are added to the wells,and any Dkk3 / REIC present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-Dkk3 polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of Dkk3 / REIC present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.
Human DKK3 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Human DKK3 ELISA Pair Set Standard Curve
Human DKK3 / Dkk-3 / REIC ELISA standard curve
DKK3 Background

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

Human DKK3 References
  • Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
  • Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
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    • Human DKK3 / Dkk-3 / REIC ELISA standard curve