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Human Endoglin / CD105 / ENG ELISA Pair Set

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Human CD105/ENG Materials provided
Capture Ab:0.5 mg/mL of mouse anti-CD105 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating
Detection Ab:0.5 mg/mL mouse anti-CD105 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use
Standard:Each vial contains 120 ng of recombinant CD105. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended
Streptavidin-HRP:
Human CD105/ENG Specificity
Human CD105/ENG Sensitivity
The minimum detectable dose of human Endoglin (CD105 / ENG) was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CD105/ENG Principle of the product
The human Endoglin (CD105 / ENG) ELISA Pair Set is for the quantitative determination of human Endoglin .
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Endoglin ( CD105 / ENG) coated on a 96-well plate. Standards and samples are added to thewells, and any Endoglin / CD105 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-Endoglin /CD105 monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of Endoglin / CD105 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
Human CD105/ENG Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human CD105/ENG ELISA Pair Set Standard Curve
Human Endoglin / CD105 / ENG ELISA standard curve
Endoglin/CD105  Background

Endoglin, also known as CD105, is a type I  homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"