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Human CD31 / PECAM-1 ELISA Pair Set

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Human CD31/PECAM1 Materials provided
Capture Ab:0.5 mg/mL of mouse anti-CD31 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating
Detection Ab:0.5 mg/mL of mouse anti-CD31 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 2.0 μg/mL in detection antibody dilution buffer before usee
Standard:Each vial contains 600 ng of recombinant CD31. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.
Human CD31/PECAM1 Specificity
Human CD31/PECAM1 Sensitivity
The minimum detectable dose of human CD31 / PECAM-1 was determined to be approximately 156.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CD31/PECAM1 Principle of the product
The human CD31 / PECAM-1 ELISA Pair Set is for the quantitative determinationof human CD31.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for human CD31 / PECAM-1 coated on a 96-well plate. Standards and samples are added to thewells, and any CD31 / PECAM-1 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-CD31monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of CD31 / PECAM-1 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
Human CD31/PECAM1 Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD31/PECAM-1 Background

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Human CD31/PECAM-1 References
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
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    Catalog: SEK10148-5
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    • Human CD31 / PECAM-1 ELISA standard curve