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Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

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Influenza HA Materials provided
Capture Ab:0.5 mg/mL of mouse anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL of rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 50 ng of recombinant Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.
Streptavidin-HRP:
Influenza HA Specificity
The following Hemagglutinin of different influenza virus types and subtypes prepared at 200 ng/mL were tested and No cross-reactivity was identified:
H1N1 (A/Brisbane/59/2007)
H5N1 (A/Anhui/1/2005)
H5N1 (A/Vietnam/1194/2004)
H3N2 (A/Brisbane/10/2007)
Influenza B (B/Florida/4/2006)
Influenza HA Sensitivity
The minimum detectable dose of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA was determined to be approximately 18.75 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Influenza HA Principle of the product
The Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set is for the quantitative determination of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA coated on a 96-well plate. Standards and samples are added to the wells, and any Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Influenza HA Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Influenza HA ELISA Pair Set Standard Curve
Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set
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Catalog: SEK001-5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"