|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat F3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80407-ACG|
|Rat F3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80407-ACR|
|Rat F3 ORF mammalian expression plasmid, C-Flag tag||RG80407-CF|
|Rat F3 ORF mammalian expression plasmid, C-His tag||RG80407-CH|
|Rat F3 ORF mammalian expression plasmid, C-Myc tag||RG80407-CM|
|Rat F3 ORF mammalian expression plasmid, C-HA tag||RG80407-CY|
|Rat F3 ORF mammalian expression plasmid, N-Flag tag||RG80407-NF|
|Rat F3 ORF mammalian expression plasmid, N-His tag||RG80407-NH|
|Rat F3 ORF mammalian expression plasmid, N-Myc tag||RG80407-NM|
|Rat F3 ORF mammalian expression plasmid, N-HA tag||RG80407-NY|
|Rat F3 natural ORF mammalian expression plasmid||RG80407-UT|
|Learn more about expression Vectors|
Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.