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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CD6 ORF mammalian expression plasmid, C-GFPSpark tag||RG80311-ACG|
|Rat CD6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80311-ACR|
|Rat CD6 ORF mammalian expression plasmid, C-Flag tag||RG80311-CF|
|Rat CD6 ORF mammalian expression plasmid, C-His tag||RG80311-CH|
|Rat CD6 ORF mammalian expression plasmid, C-Myc tag||RG80311-CM|
|Rat CD6 ORF mammalian expression plasmid, C-HA tag||RG80311-CY|
|Rat CD6 ORF mammalian expression plasmid, N-Flag tag||RG80311-NF|
|Rat CD6 ORF mammalian expression plasmid, N-His tag||RG80311-NH|
|Rat CD6 ORF mammalian expression plasmid, N-Myc tag||RG80311-NM|
|Rat CD6 ORF mammalian expression plasmid, N-HA tag||RG80311-NY|
|Rat CD6 natural ORF mammalian expression plasmid||RG80311-UT|
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T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.