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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CDH5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80276-ACG|
|Rat CDH5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80276-ACR|
|Rat CDH5 ORF mammalian expression plasmid, C-Flag tag||RG80276-CF|
|Rat CDH5 ORF mammalian expression plasmid, C-His tag||RG80276-CH|
|Rat CDH5 ORF mammalian expression plasmid, C-Myc tag||RG80276-CM|
|Rat CDH5 ORF mammalian expression plasmid, C-HA tag||RG80276-CY|
|Rat CDH5 ORF mammalian expression plasmid, N-Flag tag||RG80276-NF|
|Rat CDH5 ORF mammalian expression plasmid, N-His tag||RG80276-NH|
|Rat CDH5 ORF mammalian expression plasmid, N-Myc tag||RG80276-NM|
|Rat CDH5 ORF mammalian expression plasmid, N-HA tag||RG80276-NY|
|Rat CDH5 natural ORF mammalian expression plasmid||RG80276-UT|
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Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.