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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CDH2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80275-ACG|
|Rat CDH2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80275-ACR|
|Rat CDH2 ORF mammalian expression plasmid, C-Flag tag||RG80275-CF|
|Rat CDH2 ORF mammalian expression plasmid, C-His tag||RG80275-CH|
|Rat CDH2 ORF mammalian expression plasmid, C-Myc tag||RG80275-CM|
|Rat CDH2 ORF mammalian expression plasmid, C-HA tag||RG80275-CY|
|Rat CDH2 ORF mammalian expression plasmid, N-Flag tag||RG80275-NF|
|Rat CDH2 ORF mammalian expression plasmid, N-His tag||RG80275-NH|
|Rat CDH2 ORF mammalian expression plasmid, N-Myc tag||RG80275-NM|
|Rat CDH2 ORF mammalian expression plasmid, N-HA tag||RG80275-NY|
|Rat CDH2 natural ORF mammalian expression plasmid||RG80275-UT|
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Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.