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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat ALCAM ORF mammalian expression plasmid, C-GFPSpark tag||RG80221-ACG|
|Rat ALCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80221-ACR|
|Rat ALCAM ORF mammalian expression plasmid, C-Flag tag||RG80221-CF|
|Rat ALCAM ORF mammalian expression plasmid, C-His tag||RG80221-CH|
|Rat ALCAM ORF mammalian expression plasmid, C-Myc tag||RG80221-CM|
|Rat ALCAM ORF mammalian expression plasmid, C-HA tag||RG80221-CY|
|Rat ALCAM ORF mammalian expression plasmid, N-Flag tag||RG80221-NF|
|Rat ALCAM ORF mammalian expression plasmid, N-His tag||RG80221-NH|
|Rat ALCAM ORF mammalian expression plasmid, N-Myc tag||RG80221-NM|
|Rat ALCAM ORF mammalian expression plasmid, N-HA tag||RG80221-NY|
|Rat ALCAM natural ORF mammalian expression plasmid||RG80221-UT|
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Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.